3UHP
Crystal Structure of Glutamate Racemase from Campylobacter jejuni subsp. jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 31 |
| Unit cell lengths | 50.285, 50.285, 182.044 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.548 - 2.794 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uhf |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.785 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_851)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.794 | 2.800 |
| Number of reflections | 12747 | |
| <I/σ(I)> | 9.7 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.1 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2M Calcium Chloride, 1M Tris pH 8.5, 30% PEG400, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
