3UH8

N-terminal domain of phage TP901-1 ORF48

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98011
Spacegroup name	P 31
Unit cell lengths	41.060, 41.060, 54.780
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.560 - 2.300
R-factor	0.236
Rwork	0.235
R-free	0.25310
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.090
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	BUSTER (2.9.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.500
High resolution limit [Å]	2.300	2.300
Rmerge	0.068	0.142
Number of reflections	4537
<I/σ(I)>	33	27
Completeness [%]	99.1	99.5
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	Protein 33 mg/ml, 1.5 M K2HPO4/0.4 M NaH2PO4, 0.2M NaCl, 0.1 M imidazole, pH 8.0 , VAPOR DIFFUSION, SITTING DROP, temperature 298K