3UH1
Crystal Structure of Saccharopine Dehydrogenase from Saccharomyces cerevisiae with bound saccharopine and NADH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-10-29 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 43 |
| Unit cell lengths | 68.880, 68.880, 101.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.450 - 2.170 |
| R-factor | 0.18045 |
| Rwork | 0.178 |
| R-free | 0.22786 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.883 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.500 | 2.290 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.063 | 0.300 |
| Number of reflections | 25006 | |
| <I/σ(I)> | 14.5 | 4.3 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 22 % PEG 3350, 0.1 M Bis-Tris, 0.3 M Malonate, NAD and saccharopine, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
