3UGU

Crystal Structure of p44 (Splice Variant of Visual Arrestin)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9766
Spacegroup name	P 21 21 21
Unit cell lengths	66.964, 71.583, 79.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.482 - 1.850
R-factor	0.1763
Rwork	0.174
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.075
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.680	1.880
High resolution limit [Å]	1.850	1.850
Rmerge	0.069	0.299
Number of reflections	33085
<I/σ(I)>		5.3
Completeness [%]	99.2	96.6
Redundancy	6.7	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292.15	14% PEG 4000, 0.1M MES, 50 mM KH2PO4, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 292.15K