3UGE
Silver Metallated Pseudomonas aeruginosa Azurin at 1.70 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER IMUS MICROFOCUS |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-29 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.746, 97.815, 110.029 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.350 - 1.700 |
| R-factor | 0.20556 |
| Rwork | 0.203 |
| R-free | 0.24459 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e67 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.431 |
| Data reduction software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.680 | 1.730 |
| High resolution limit [Å] | 1.650 | 1.700 |
| Number of reflections | 64144 | |
| <I/σ(I)> | 10.97 | 2.04 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 7.35 | 4.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 3.2 M ammonium sulfate, 0.5 M lithium nitrate, 50 mM sodium acetate, pH 5.5, soaked with 1 mM silver nitrate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
