3UG6

Crystal Structure of Get3 from Methanocaldococcus jannaschii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	173
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	85.736, 76.874, 127.810
Unit cell angles	90.00, 108.83, 90.00

Refinement procedure

Resolution	42.864 - 3.300
R-factor	0.2506
Rwork	0.249
R-free	0.28750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.022
RMSD bond angle	1.664
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall
Low resolution limit [Å]	120.971
High resolution limit [Å]	3.292
Number of reflections	23100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	9% PEG3350, 0.1 M sodium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298K