3UG5
Crystal structure of alpha-L-arabinofuranosidase from Thermotoga maritima xylose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.200, 160.933, 156.172 |
| Unit cell angles | 90.00, 91.84, 90.00 |
Refinement procedure
| Resolution | 29.590 - 2.300 |
| R-factor | 0.16957 |
| Rwork | 0.168 |
| R-free | 0.20892 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pz3 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.738 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 221594 | |
| <I/σ(I)> | 17.6 | 2.5 |
| Completeness [%] | 99.7 | 98.7 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1M sodium citrate, 0.1M Tris-HCl, 0.2M NaCl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
