3UG0

Crystal structure of a Trp-less green fluorescent protein translated by the simplified genetic code

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-25
Detector	RAYONIX MX225HE
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	51.784, 63.050, 67.401
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.701 - 2.093
R-factor	0.1832
Rwork	0.180
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mea
RMSD bond length	0.007
RMSD bond angle	1.339
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.090	5.700	2.100
Rmerge	0.110	0.065	0.387
Number of reflections	13473
<I/σ(I)>	10.1
Completeness [%]	99.6	99.1	97.7
Redundancy	9.5	11.2	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	17% PEG 20000, 100mM MES (pH6.5), vapor diffusion, sitting drop, temperature 293K