3UFZ

Crystal structure of a Trp-less green fluorescent protein translated by the universal genetic code

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-25
Detector	RAYONIX MX225HE
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	51.844, 62.955, 67.369
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.906 - 1.850
R-factor	0.1867
Rwork	0.185
R-free	0.22770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ema
RMSD bond length	0.007
RMSD bond angle	1.364
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0066)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.880
High resolution limit [Å]	1.850	5.020	1.850
Rmerge	0.095	0.042	0.479
Number of reflections	19367
<I/σ(I)>	14.6
Completeness [%]	99.6	99.2	99.1
Redundancy	10.5	12.1	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	15% PEG 6000, 5% Glycerol, pH 8.5, vapor diffusion, sitting drop, temperature 293K