3UF5

Crystal structure of the mouse Colony-Stimulating Factor 1 (mCSF-1) cytokine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-05-08
Detector	PSI PILATUS 6M
Wavelength(s)	0.9726
Spacegroup name	P 21 21 21
Unit cell lengths	36.320, 68.690, 144.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.459 - 2.800
R-factor	0.2144
Rwork	0.210
R-free	0.25780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.545
Refinement software	PHENIX ((phenix.refine: dev_874))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	9314
Completeness [%]	98.7	98.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1M Tris, 20% PEG 6000, 0.2M calcium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K