3UF4

Crystal structure of a SH3 and SH2 domains of FYN protein (Proto-concogene Tyrosine-protein kinase Fyn) from Mus musculus at 1.98 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-08
Detector	ADSC QUANTUM 315
Spacegroup name	C 1 2 1
Unit cell lengths	138.220, 35.960, 43.960
Unit cell angles	90.00, 93.56, 90.00

Refinement procedure

Resolution	43.876 - 1.980
R-factor	0.2007
Rwork	0.200
R-free	0.22430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	XDS
Data scaling software	XSCALE (December 6, 2010)
Phasing software	MOLREP
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.876		2.050
High resolution limit [Å]	1.980	4.250	1.980
Rmerge	0.058	0.028	0.811
Number of reflections	14960	1616	1420
<I/σ(I)>	15.73	42.5	1.8
Completeness [%]	97.8	99.5	96.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	4.3M NaCl, 0.1M HEPES pH 7.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K