3UEQ

Crystal structure of amylosucrase from Neisseria polysaccharea in complex with turanose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-06
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9334
Spacegroup name	P 21 21 2
Unit cell lengths	96.030, 116.270, 60.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.070 - 1.850
R-factor	0.14911
Rwork	0.147
R-free	0.18564
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g5a
RMSD bond length	0.022
RMSD bond angle	1.757
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.270	1.950
High resolution limit [Å]	1.850	1.850
Number of reflections	55312
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	285	30% PEG 6000, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 285.0K