3UEI
Crystal structure of human Survivin E65A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.561, 71.213, 83.203 |
| Unit cell angles | 90.00, 129.66, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.17925 |
| Rwork | 0.177 |
| R-free | 0.22823 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uec |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.682 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.084 | 0.556 |
| Number of reflections | 13904 | |
| <I/σ(I)> | 29.8 | 2.4 |
| Completeness [%] | 96.9 | 94.1 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 5% Tacsimate, 0.1 M HEPES-Na, 12% PEG monomethyl eter, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
