3UED
Crystal structure of human Survivin bound to histone H3 phosphorylated on threonine-3 (C2 space group).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.415, 71.276, 83.136 |
| Unit cell angles | 90.00, 129.91, 90.00 |
Refinement procedure
| Resolution | 41.280 - 2.700 |
| R-factor | 0.19469 |
| Rwork | 0.192 |
| R-free | 0.24086 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uec |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.636 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.720 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.055 | 0.480 |
| Number of reflections | 14102 | |
| <I/σ(I)> | 32.3 | 2.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 15% PEG 3350, 0.16 M sodium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
