3UEB
Crystal structure of TON_0450 from Thermococcus onnurineus NA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2011-02-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 108.059, 108.059, 127.603 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.750 - 1.980 |
| Rwork | 0.206 |
| R-free | 0.25270 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.194 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.750 |
| High resolution limit [Å] | 1.980 |
| Number of reflections | 73191 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 298 | 0.1M Bis-Tris (PH5.3), 22% PEG 3350, 0.22M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
