3UDS
Inositol 1,3,4,5,6-pentakisphosphate 2-kinase from A. thaliana in complex with ADP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-07-31 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.289, 59.877, 82.913 |
| Unit cell angles | 83.40, 89.40, 65.30 |
Refinement procedure
| Resolution | 42.820 - 3.100 |
| R-factor | 0.2408 |
| Rwork | 0.237 |
| R-free | 0.31190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.319 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.6.3_473) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.650 |
| Number of reflections | 27584 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 0.1M Tris-HCl, 0.18M CaCl2, 18% PEG6000, 0.01M TCEP, Protein: 10mg/mL, Temp: 293K, pH 8.0, vapor diffusion |
