3UDA
Crystal Structure Analysis of FGF1-Disaccharide(NI24) complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 113.630, 45.981, 96.558 |
Unit cell angles | 90.00, 125.68, 90.00 |
Refinement procedure
Resolution | 78.430 - 2.510 |
R-factor | 0.21418 |
Rwork | 0.207 |
R-free | 0.27642 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.475 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | EPMR |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.430 | 2.590 |
High resolution limit [Å] | 2.510 | 2.510 |
Rmerge | 0.058 | 0.288 |
Number of reflections | 13268 | |
<I/σ(I)> | 11.8 | 2.5 |
Completeness [%] | 93.6 | 81.4 |
Redundancy | 2.9 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2M (NH4)HPO4, 0.1M sodium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |