3UCS
Crystal structure of the complex between CBPA J-domain and CBPM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-24 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.816, 77.094, 111.231 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.870 |
| R-factor | 0.1977 |
| Rwork | 0.196 |
| R-free | 0.23170 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.150 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.940 |
| High resolution limit [Å] | 1.870 | 4.030 | 1.870 |
| Rmerge | 0.062 | 0.030 | 0.489 |
| Number of reflections | 38222 | ||
| <I/σ(I)> | 14.8 | ||
| Completeness [%] | 99.7 | 98.8 | 98.7 |
| Redundancy | 8.1 | 7.8 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1M HEPES pH 7.0, 20% PEG 8000, vapor diffusion, hanging drop, temperature 277K |
