3UBG
Crystal structure of Drosophila N-cadherin EC1-3, II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-21 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9191 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.218, 113.769, 87.872 |
| Unit cell angles | 90.00, 123.57, 90.00 |
Refinement procedure
| Resolution | 19.973 - 2.502 |
| R-factor | 0.1992 |
| Rwork | 0.196 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.083 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 30014 | |
| <I/σ(I)> | 12.4 | 2.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 34% PEG 3350, 0.1M lithium sulfate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
