3UB9

Periplasmic portion of the Helicobacter pylori chemoreceptor TlpB with hydroxyurea bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	79.611, 81.539, 94.554
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.650 - 1.420
R-factor	0.1338
Rwork	0.133
R-free	0.15670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.593
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.450
High resolution limit [Å]	1.420	3.500	1.420
Rmerge	0.062	0.048	0.394
Number of reflections	113775
<I/σ(I)>	13.1
Completeness [%]	97.6	94.8	91.5
Redundancy	3.5	3.6	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.1 M Bis-Tris pH 7.0, 2.0 M ammonium sulfate, vapor diffusion, hanging drop, temperature 277K