3UB8
Periplasmic portion of the Helicobacter pylori chemoreceptor TlpB with formamide bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.610, 81.713, 94.538 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.830 - 1.420 |
| R-factor | 0.14 |
| Rwork | 0.139 |
| R-free | 0.16500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.492 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.450 |
| High resolution limit [Å] | 1.420 | 3.500 | 1.420 |
| Rmerge | 0.052 | 0.041 | 0.302 |
| Number of reflections | 92912 | ||
| <I/σ(I)> | 14.8 | ||
| Completeness [%] | 79.6 | 68.3 | 80 |
| Redundancy | 4.2 | 5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1 M Bis-Tris pH 6.5, 2.1 M ammonium sulfate, vapor diffusion, hanging drop, temperature 277K |
