3UAI
Structure of the Shq1-Cbf5-Nop10-Gar1 complex from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-06 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 100.995, 100.995, 272.329 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.060 |
| R-factor | 0.22162 |
| Rwork | 0.219 |
| R-free | 0.27526 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uah 3u28 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.210 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.060 | 3.060 |
| Rmerge | 0.067 | 0.684 |
| Number of reflections | 27487 | |
| <I/σ(I)> | 58.4 | 5.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.1 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 297 | 0.1M Bis-Tris, 20%(w/v) polyethylene glycol monomethyl ether 5000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
