3UAG
UDP-N-ACETYLMURAMOYL-L-ALANINE:D-GLUTAMATE LIGASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 |
| Unit cell lengths | 65.240, 65.240, 134.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.770 |
| Rwork | 0.178 |
| R-free | 0.22200 |
| Structure solution method | OTHER |
| RMSD bond length | 0.012 |
| RMSD bond angle | 25.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.900 | 1.830 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.066 * | 0.204 * |
| Number of reflections | 54268 | |
| <I/σ(I)> | 25.6 | 8.6 |
| Completeness [%] | 99.2 | 98.7 |
| Redundancy | 4.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9.8 (mg/ml) | |
| 2 | 1 | drop | UMA | 1 (mM) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | HEPES | 20 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 1.6-1.8 (M) | |
| 7 | 1 | reservoir | HEPES | 100 (mM) |
