3UAF

Crystal Structure of a TTR-52 mutant of C. elegans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2007-08-08
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	70.321, 33.368, 49.265
Unit cell angles	90.00, 106.83, 90.00

Refinement procedure

Resolution	33.655 - 2.010
R-factor	0.1776
Rwork	0.173
R-free	0.22140
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	1.105
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.080
High resolution limit [Å]	2.010	4.330	2.010
Number of reflections	7360
Completeness [%]	97.6	99.6	97.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.5	289	0.2M ammonium sulfate, 0.1M soldium acetate, 35% PEGME2000, pH 4.5, EVAPORATION, temperature 289K
1	EVAPORATION	4.5	289	0.2M ammonium sulfate, 0.1M soldium acetate, 35% PEGME2000, pH 4.5, EVAPORATION, temperature 289K