3UA0
N-Terminal Domain of Bombyx mori Fibroin Mediates the Assembly of Silk in Response to pH Decrease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Detector technology | CCD |
| Collection date | 2009-10-07 |
| Detector | RAYONIX MX225HE |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 74.520, 74.520, 208.230 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.260 - 3.000 |
| R-factor | 0.312 |
| Rwork | 0.311 |
| R-free | 0.33600 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.145 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.112 | 0.654 |
| Number of reflections | 7427 | |
| <I/σ(I)> | 40.2 | 3.3 |
| Completeness [%] | 99.4 | 94.5 |
| Redundancy | 31.9 | 19.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 1.2M (NH4)2SO4, 0.1M SODIUM ACETATE PH 4.6, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 289K |
