3U9J

Crystal structure of oxidized human FBXL5 hemerythrin like domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	120
Detector technology	CCD
Collection date	2011-05-13
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 21 21 2
Unit cell lengths	55.082, 77.343, 78.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.881 - 1.603
R-factor	0.1689
Rwork	0.167
R-free	0.21740
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.754
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.7_650)
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.000	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	44325
Completeness [%]	99.4	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	15% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K