3U9G
Crystal structure of the Zinc finger antiviral protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.25 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 52.894, 52.894, 138.275 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.491 - 1.801 |
| R-factor | 0.1929 |
| Rwork | 0.191 |
| R-free | 0.22820 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.938 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.324 | |
| Number of reflections | 21576 | |
| <I/σ(I)> | 30.4 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 19.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 15% (w/v) PEG 3350, 0.06M magnesium formate dehydrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
