3U9F
Structure of CATI in complex with chloramphenicol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 107.336, 114.429, 114.374 |
| Unit cell angles | 119.97, 97.83, 98.64 |
Refinement procedure
| Resolution | 35.000 - 2.900 |
| R-factor | 0.2401 |
| Rwork | 0.236 |
| R-free | 0.30937 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.909 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.900 |
| Number of reflections | 89861 |
| Completeness [%] | 87.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 100 mM HEPES pH 7.5, 20% PEG 4000, isopropanol 10% v/v, reservoir solution contains 1 mM chloramphenicol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
