3U92
Crystal structure of the GluK3 ligand binding domain complex with kainate and zinc: P2221 form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-07 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 129.537, 55.654, 90.052 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.530 - 1.900 |
R-factor | 0.1887 |
Rwork | 0.187 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s50 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.043 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.053 | 0.589 |
Number of reflections | 52357 | |
<I/σ(I)> | 34 | 2.9 |
Completeness [%] | 98.8 | 95.9 |
Redundancy | 7.3 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 5% PEG 8K, 0.1 M Tris, 4 mM Kainate, 2 mM ZnAcetate, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |