3U91
Structural Determinants of Trimerization Specificity in HIV-1 gp41 Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9676 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 45.308, 45.308, 42.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.140 - 1.450 |
| R-factor | 0.19585 |
| Rwork | 0.192 |
| R-free | 0.23341 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aik |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.138 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.140 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.065 | |
| Number of reflections | 9173 | |
| <I/σ(I)> | 9.1 | 6.4 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 8.8 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 20% Jeffamine M600, 0.1M MES, 25mM Cesium Chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
