3U8X
Crystal Structure of a chimera containing the N-terminal domain (residues 8-29) of drosophila Ciboulot and the C-terminal domain (residues 18-44) of bovine Thymosin-beta4, bound to G-actin-ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97903 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.011, 75.326, 128.866 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.700 - 2.000 |
R-factor | 0.19 |
Rwork | 0.186 |
R-free | 0.23820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sqk |
RMSD bond length | 0.025 |
RMSD bond angle | 2.276 |
Data scaling software | XSCALE |
Phasing software | PHASER (1.3.2) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.010 | |
High resolution limit [Å] | 2.000 | 15.000 | 2.000 |
Rmerge | 0.100 | 0.055 | 0.445 |
Number of reflections | 59601 | 83 | 790 |
<I/σ(I)> | 17.06 | 20.51 | 4.23 |
Completeness [%] | 99.1 | 53.2 | 88.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 6.5 | 298 | 20% PEG3350, 0.2M MgAcetate pH6.5, 0.45M Guanidine HCl, 1% Dioxane, hanging drop, temperature 298K |