Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2007-10-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0, 1.48525, 1.48634, 1.40127 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.660, 56.207, 83.923 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.680 - 1.900 |
| R-factor | 0.17206 |
| Rwork | 0.170 |
| R-free | 0.20533 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.638 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 13646 | |
| <I/σ(I)> | 10.5 | 2.6 |
| Completeness [%] | 98.5 | 97.6 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 289 | The reservoir contained 20% w/v polyethylene glycol 20000, 0.1 M calcium chloride, 0.1M N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid, TAPS and 1 mM sodium azide. For crystallization, 1 UL of the protein was mixed with an equal volume of the reservoir. The total volume of the reservoir was 0.5 ml., pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
