Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2003-12-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 32.136, 25.172, 34.094 |
| Unit cell angles | 90.00, 116.25, 90.00 |
Refinement procedure
| Resolution | 16.060 - 0.960 |
| R-factor | 0.10463 |
| Rwork | 0.103 |
| R-free | 0.12487 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bd1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.984 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.600 |
| High resolution limit [Å] | 0.910 |
| Rmerge | 0.079 |
| Number of reflections | 14269 |
| <I/σ(I)> | 53.4 |
| Completeness [%] | 80.6 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | Sodium Cacodylate, Magnesium Acetate, MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
