3U86

Crystal structure of human menin in complex with JunD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-10-15
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.98019
Spacegroup name	P 41 21 2
Unit cell lengths	140.647, 140.647, 93.106
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.984 - 2.843
R-factor	0.2195
Rwork	0.217
R-free	0.24510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.032
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	2.950
High resolution limit [Å]	2.843	6.140	2.850
Rmerge	0.100	0.063	0.446
Number of reflections	21430
<I/σ(I)>	12.8
Completeness [%]	95.0	98.4	75.1
Redundancy	11.5	11	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	2.3 M NaCl, pH 7.0, vapor diffusion, sitting drop, temperature 277K