3U7V
The structure of a putative Beta-galactosidase from Caulobacter crescentus CB15.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97912 |
| Spacegroup name | I 4 |
| Unit cell lengths | 148.306, 148.306, 53.792 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.400 - 1.800 |
| R-factor | 0.1854 |
| Rwork | 0.184 |
| R-free | 0.21470 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.515 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
| Rmerge | 0.116 | 0.090 | 0.398 |
| Number of reflections | 54354 | ||
| <I/σ(I)> | 7.8 | ||
| Completeness [%] | 100.0 | 99.5 | 100 |
| Redundancy | 7.8 | 7.2 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 297 | 0.17 Amonium sulfate, 0.085 sodium citrate: HCl pH 5.6, 25.5% PEG 4K, 15% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
