3U79
AL-103 Y32F Y96F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-04-27 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 128.020, 128.020, 98.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.060 - 1.620 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.025 |
| RMSD bond angle | 0.814 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.000 | 1.690 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Number of reflections | 112286 | |
| Completeness [%] | 95.4 | 77.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.21 | 277 | 20% (w/v) polyethylene glycol monomethyl ether and 0.2M Sodium Acetate in 0.1 M MES buffer (pH 8.21), VAPOR DIFFUSION, HANGING DROP, temperature 277K |
