3U6X
Phage TP901-1 baseplate tripod
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-26 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 149.960, 237.420, 152.210 |
| Unit cell angles | 90.00, 115.67, 90.00 |
Refinement procedure
| Resolution | 49.740 - 2.600 |
| R-factor | 0.1812 |
| Rwork | 0.178 |
| R-free | 0.20640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ejc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.079 | 0.480 |
| Number of reflections | 298571 | |
| <I/σ(I)> | 10.3 | 2.1 |
| Completeness [%] | 98.1 | 98.4 |
| Redundancy | 2.5 | 2.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 273 | Concentration of tripod: 5.3 mg/ml, 25% PEG1000, 0.1 M HEPES pH 7.2, , VAPOR DIFFUSION, SITTING DROP, temperature 273K |
