3U66

Crystal structure of T6SS SciP/TssL from Escherichia Coli Enteroaggregative 042

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Detector technology	CCD
Collection date	2011-08-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	I 2 2 2
Unit cell lengths	51.610, 82.030, 96.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.680 - 2.630
R-factor	0.2375
Rwork	0.235
R-free	0.25730
Structure solution method	SAD
RMSD bond length	0.000
RMSD bond angle	0.000
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	BUSTER (2.9.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.700
High resolution limit [Å]	2.630	2.630
Rmerge	0.056	0.600
Number of reflections	11601
<I/σ(I)>	20.2	2.5
Completeness [%]	99.4	99.8
Redundancy	5.7	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.6	273	mixing 300 nL protein at 23mg/mL with 100 nL 4.3 M NaCl, 0.1 M Bicine pH 10.6. , VAPOR DIFFUSION, SITTING DROP, temperature 273K
1	VAPOR DIFFUSION, SITTING DROP	10.6	273	mixing 300 nL protein at 23mg/mL with 100 nL 4.3 M NaCl, 0.1 M Bicine pH 10.6. , VAPOR DIFFUSION, SITTING DROP, temperature 273K