3U64
The Crystal Structure of Tat-T (Tp0956)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97758 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 127.078, 127.078, 127.078 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.953 - 2.300 |
| R-factor | 0.1989 |
| Rwork | 0.197 |
| R-free | 0.24160 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.006 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
| Rmerge | 0.077 | 0.034 | 0.899 |
| Number of reflections | 15399 | ||
| <I/σ(I)> | 16.3 | ||
| Completeness [%] | 99.9 | 99.6 | 99.7 |
| Redundancy | 19.2 | 19.9 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9 | 293 | 30% PEG-400, 0.1 M Bicine, 0.2 M MgCl2, pH 9.0, vapor diffusion, temperature 293K |
