3U59
N-terminal 98-aa fragment of smooth muscle tropomyosin beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.121, 75.711, 137.637 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.000 - 2.500 |
| R-factor | 0.33465 |
| Rwork | 0.295 |
| R-free | 0.33667 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.416 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.071 | 0.505 |
| Number of reflections | 20734 | |
| <I/σ(I)> | 15.82 | 2.98 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 7.2 | 7.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.1 M HEPES, 5 mM CaCl2, 28-31 % 2- methyl-2,4-pentanediol, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.4 |
