3U4V

Crystal Structure of the Tetrahymena telomerase processivity factor Teb1 OB-A

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-02
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97887, 0.97926
Spacegroup name	P 41
Unit cell lengths	67.746, 67.746, 70.061
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.952 - 1.800
R-factor	0.1819
Rwork	0.181
R-free	0.19960
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.018
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	1.830
High resolution limit [Å]	1.800	4.890	1.800
Rmerge	0.133	0.068	0.428
Number of reflections	29408
<I/σ(I)>	9.1
Completeness [%]	99.8	98.8	98
Redundancy	13.6	13.7	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	277	3 M NaCl, pH 4.8, vapor diffusion, hanging drop, temperature 277K