3U4T
Crystal Structure of the C-terminal part of the TPR repeat-containing protein Q11TI6_CYTH3 from Cytophaga hutchinsonii. Northeast Structural Genomics Consortium Target ChR11B.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97898 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.671, 42.822, 104.946 |
| Unit cell angles | 90.00, 92.83, 90.00 |
Refinement procedure
| Resolution | 42.284 - 2.282 |
| R-factor | 0.239 |
| Rwork | 0.238 |
| R-free | 0.25600 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.380 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.282 | 2.300 |
| Rmerge | 0.095 | 0.345 |
| Number of reflections | 62017 | |
| <I/σ(I)> | 12.4 | 2.3 |
| Completeness [%] | 93.6 | 91.2 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch crystallization under oil | 8 | 291 | 20% PEG 8000, 0.1M ammonium phosphate, 0.1M Tris-HCl, pH 8.0, Microbatch crystallization under oil, temperature 291K |
