3U4Q
Structure of AddAB-DNA complex at 2.8 angstroms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.555, 139.714, 103.093 |
| Unit cell angles | 90.00, 105.54, 90.00 |
Refinement procedure
| Resolution | 29.918 - 2.800 |
| R-factor | 0.2358 |
| Rwork | 0.233 |
| R-free | 0.28650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.655 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: dev_750)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.500 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 64678 | |
| Completeness [%] | 95.7 | 95.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285 | 15% polyethylene glycol 4000, 0.1M Tris pH 7.5, 0.8 M sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
