3U46

CH04H/CH02L P212121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-07-22
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.00
Spacegroup name	P 21 2 21
Unit cell lengths	70.607, 105.539, 163.976
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.373 - 2.906
R-factor	0.2162
Rwork	0.215
R-free	0.24480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.582
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	22893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	16% PEG 400, 8% PEG 8000, 0.1M Acetate pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K