3U44
Crystal structure of AddAB-DNA complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.230, 138.960, 102.970 |
| Unit cell angles | 90.00, 105.33, 90.00 |
Refinement procedure
| Resolution | 29.884 - 3.201 |
| R-factor | 0.2351 |
| Rwork | 0.233 |
| R-free | 0.28390 |
| Structure solution method | MAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.711 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: dev_750)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 44552 | |
| Completeness [%] | 99.0 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285 | 15% polyethylene glycol 4000, 0.1M Tris pH 7.5, 0.8 M sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
