3U3T

The S-SAD phased crystal structure of the ecto-domain of Death Receptor 6 (DR6)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-11
Detector	ADSC QUANTUM 315
Wavelength(s)	2.7
Spacegroup name	P 61 2 2
Unit cell lengths	77.655, 77.655, 186.459
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 3.210
R-factor	0.21041
Rwork	0.208
R-free	0.26078
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.892
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.210
Number of reflections	5655
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2M ammonium acetate, 0.1M sodium citrate tribasic dehydrate (pH5.0-6.0), 25-30% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 289K