3U3G
Structure of LC11-RNase H1 Isolated from Compost by Metagenomic Approach: Insight into the Structural Bases for Unusual Enzymatic Properties of Sto-RNase H1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.315, 104.891, 57.212 |
| Unit cell angles | 90.00, 110.34, 90.00 |
Refinement procedure
| Resolution | 47.760 - 1.400 |
| R-factor | 0.17319 |
| Rwork | 0.172 |
| R-free | 0.19204 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h08 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.061 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.066 | 0.451 |
| Number of reflections | 115103 | |
| <I/σ(I)> | 31.7 | 2.8 |
| Completeness [%] | 97.4 | 85.8 |
| Redundancy | 7 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 0.1M imidazole, 0.2M sodium cloride, 0.4M sodium dihydrogen phosphate, 1.6M dipotassium phosphate, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
