3U35
Crystal structure of the general stress FMN/FAD binding protein from the phytopathogen Xanthomonas citri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.116 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 120.204, 122.184, 57.713 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.805 - 2.500 |
| R-factor | 0.2031 |
| Rwork | 0.201 |
| R-free | 0.23920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.035 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.066 | 0.653 |
| Number of reflections | 29232 | |
| <I/σ(I)> | 24 | 2.3 |
| Completeness [%] | 97.0 | 98.6 |
| Redundancy | 4.8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | Protein concentration at 25mg/mL in 0.1M Hepes, pH 7.5, 42% PEG200, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
