3U34

Crystal structure of the general stress FMN/FAD binding protein from the phytopathogen Xanthomonas citri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-02-03
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.541870
Spacegroup name	P 21 21 2
Unit cell lengths	120.308, 122.135, 57.734
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.807 - 2.800
R-factor	0.2147
Rwork	0.212
R-free	0.26020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dmb
RMSD bond length	0.012
RMSD bond angle	1.314
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	20973
Completeness [%]	96.9	95.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	Protein concentration at 25 mg/mL in 0.1M Hepes buffer, pH 7.5, containing 42% PEG200, VAPOR DIFFUSION, SITTING DROP, temperature 298K