3U31
Plasmodium falciparum Sir2A preferentially hydrolyzes medium and long chain fatty acyl lysine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.97 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 32.173, 102.734, 105.184 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.2046 |
| Rwork | 0.202 |
| R-free | 0.24650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jwp |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.066 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
| Rmerge | 0.129 | 0.079 | 0.748 |
| Number of reflections | 18544 | ||
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 99.9 | 98.6 | 99.9 |
| Redundancy | 6.7 | 5.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 16% PEG 3350, 0.1M NaF, 7% Formamide, vapor diffusion, hanging drop, temperature 298K |
